Dr. Yizhi Meng, an assistant professor and researcher for Stony Brook University, could usually be found working in the Heavy Engineering Building. She stumbled across iCREATE in Fall 2016 and was able to produce a few custom-designed well plates for use in her laboratory.
Dr. Meng studies how to engineer 3-D micro-tumors of 50,000-80,000 cells, also known as “cancer on a chip”. As described in her bio, “seeks to study new approaches to developing bio-inspired materials and strategies for tissue engineering”.
What does that mean?
By growing multiple micro-tumors at once, you could perform drug screenings of cancer drugs. Using micro-tumors keeps it as realistic as possible, instead of animal studies. This in-vitro model is less expensive, removes animal variation as a potential error, and allows the trial and test of hundreds of drugs at a time. In short, they aim to create a better target to test different types of drugs, and this requires constant use of a lot of well plates to grow cancer cells on.
This is crucial because of the way these micro-tumors and cultures behave on a hard plastic dish. Even though this is the way we have been studying them, this is not how they would behave when they are in the body. The purpose of the lab is to test these cells in a safe environment where unintentional effects are not exaggerated.
Traditional trays with these wells are made with a hydrophobic polystyrene, a waterproof hard plastic, with a flat bottom. While these can easily be bought from about any laboratory item supplier, the bottom of the wells make it a slippery surface for the cells to grasp onto and they often slip in transport. “This sliding interferes with imaging, and that leads to other difficulties” says Dr. Meng.
Dr. Meng heard of a 3-D printing service on campus through other staff members. At a strong STEM research campus like Stony Brook University, 3-D printers are not unheard of, however, almost all of them are for private departmental or laboratory access use only. Dr. Meng got in contact and came to iCREATE director David Ecker with a question: Was it possible to produce a tangible and simple solution?
Giancarlos, one of our iCREATE staff members, assisted with Dr. Meng and her laboratory staff. After several iterations of working out and rendering the details and dimensions of the file, the print of the trays took about one day to complete in our iCREATE facility, using the Form 2 3-D printer. This printer is unique from the others because it uses resin to produce a detailed, denser, and smoother end product.
There were two versions designed that differed by the shape on the bottom. One was ice-cream cone shaped, and the other was like rounded hemisphere. These little indentations allow the cells to stay stable in the center during transport without sliding.
Above: This well plate has the ice cream cone shaped bottom.
Above: This well plate has the rounded hemisphere shaped bottom.
Currently, the resin material that the trays are printed with is still being tested for its biocompatibility but so far, Dr. Meng says they have been working well. “The material is not killing them, and the cells are healthy. It appears to be biologically inert.” The well plates can be sterilized for reuse when needed.
“Now, I have so many ideas of what other things to try to make,” Dr. Meng said, “It’s like bypassing the big suppliers. An idea can move from a CAD file to a physical object.”
From left to right: Giancarlos, Dr, Meng, and her graduate student lab assistant Weiyi Li
Dr. Meng (left) with Weiyi Li (center) and Vincent Alford (right). Weiyi is a graduate student in the Department of Materials Science and Chemical Engineering, and Vincent is a graduate student in Molecular and Cellular Pharmacology.
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Dr. Yizhi Meng is a professor at Stony Brook University. She found out about iCREATE in 2016 and used it to make special plates for her lab work. She studies how to create tiny 3-D tumors with 50,000-80,000 cells, like “cancer on a chip.” Her goal is to make materials for growing tissues in new ways.
I find Dr. Meng’s work really interesting. It makes me think about “Hamza Estimate.” This place has useful ideas about similar things. It can help us understand more about growing tissues and the techniques used. If you’re curious like me, checking out Dr. Meng’s research and “Hamza Estimate ” could teach us a lot.
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