Undergraduate Research at SUNY Purchase
My project is centered on analyzing Renibacterium salmoninarum, a bacterium that infects the kidneys of salmon and causes significant losses in aquaculture. I worked with both R. salmoninarum and Aeromonas salmonicida, another pathogen affecting salmon. My approach involved using PMAxx dye to differentiate between live and dead cells in PCR assays, enhancing diagnostic precision by selectively binding to the DNA of dead cells, thereby inhibiting its amplification. Through this work, I became skilled in advanced PCR techniques, gel electrophoresis, and DNA isolation protocols, adapting these techniques to produce reliable and accurate results.
In this project, I also prepared specialized agar and growth media for culturing bacteria, using charcoal agar for R. salmoninarum to optimize bacterial growth. For detection, I used primers targeting the p57 gene for R. salmoninarum and the vapA gene for A. salmonicida, enabling me to confirm virulent strains. My methods involved creating master mixes for PCR and running multiple rounds of gel electrophoresis to confirm my results, demonstrating proficiency in setting up, troubleshooting, and interpreting molecular assays.
Alongside my molecular research, I also conducted a dissection of a dogfish shark, where I located complex anatomical structures like the brain and optic lobe. This experience required precise handling of dissection tools and reinforced my knowledge of comparative anatomy, as the brain was difficult to locate due to the thick cartilage surrounding it. This hands-on work developed my anatomical skills and allowed me to connect theoretical knowledge with practical application.
Additionally, my role as a lab assistant broadened my research skills as I gained experience in lab management, assisted peers in complex procedures, and refined my technical abilities in molecular biology. This experience solidified my passion for research and allowed me to make impactful contributions to pathogen detection methods, setting a strong foundation for my future work in biomedical research.
Ongoing Graduate Research at Stony Brook University
In my graduate studies at Stony Brook University, I am working in Prof. Shufeng’s lab, focusing on innovative drug delivery systems aimed at treating chronic diseases, particularly inflammatory bowel disease (IBD). My work is centered on developing hydrogels that utilize ascorbyl palmitate to self-assemble into microfibers, which adhere to inflamed gastrointestinal tissues. These hydrogels are designed to deliver anti-inflammatory drugs, such as dexamethasone, directly to affected areas, with the aim of enhancing drug retention and release specificity. Through this project, I’ve gained experience in polymer chemistry and the formulation of drug-delivery vehicles tailored to target specific sites within the body.
Additionally, I am exploring the potential of nanoparticle (NP) systems to target inflamed tissues in the gastrointestinal tract with greater precision. I am investigating human serum albumin-coated nanoparticles, paired with heparin, which can bind to positively charged proteins common in inflamed areas, improving targeting accuracy. My work involves optimizing the size and coating of these NPs to enhance their adhesion and therapeutic impact, especially for combination drug therapies. This research has refined my expertise in nanoparticle engineering, particle characterization, and targeted delivery mechanisms, all of which are vital in advancing treatment options for chronic diseases.
In both the hydrogel and nanoparticle projects, I employ various characterization techniques, such as dynamic light scattering (DLS) and scanning electron microscopy (SEM), to assess particle size, distribution, and surface morphology. These skills are crucial for ensuring the stability and effectiveness of the delivery systems, as well as for troubleshooting and refining formulations. Through this research, I aim to contribute to more effective and patient-specific treatments that reduce the impact of chronic inflammatory diseases, thus improving patient outcomes and quality of life.