Publications

  1. J. L. Coleman, J. L. Benach, A. W. Karzai, Endogenous and Borrowed Proteolytic Activity in the Borrelia. Microbiol Mol Biol Rev85, (2021).
  2. M. Shin et al., Structural basis for distinct operational modes and protease activation in AAA+ protease Lon. Sci Adv6, eaba8404 (2020).
  3. N. Puri, A. W. Karzai, HspQ Functions as a Unique Specificity-Enhancing Factor for the AAA+ Lon Protease. Mol Cell66, 672-683 e674 (2017).
  4. L. R. Friedlander, N. Puri, M. A. Schoonen, A. Wali Karzai, The effect of pyrite on Escherichia coli in water: proof-of-concept for the elimination of waterborne bacteria by reactive minerals. J Water Health13, 42-53 (2015).
  5. K. Venkataraman, H. Zafar, A. W. Karzai, Distinct tmRNA sequence elements facilitate RNase R engagement on rescued ribosomes for selective nonstop mRNA decay. Nucleic Acids Res, (2014).
  6. K. Venkataraman, K. E. Guja, M. Garcia-Diaz, A. W. Karzai, Non-stop mRNA decay: a special attribute of trans-translation mediated ribosome rescue. Front Microbiol5, 93 (2014).
  7. N. P. Lonia R. Friedlander, Martin A. A. Schoonen and A. Wali Karzai, The effect of pyrite on Escherichia coli in water: proof-of-concept for the elimination of waterborne bacteria by reactive minerals. Journal of Water and Health, (2014).
  8. K. E. Guja et al., Structural basis for S-adenosylmethionine binding and methyltransferase activity by mitochondrial transcription factor B1. Nucleic Acids Res41, 7947-7959 (2013).
  9. D. Camenares, D. P. Dulebohn, A. Svetlanov, A. W. Karzai, Active and accurate trans-translation requires distinct determinants in the C-terminal tail of SmpB protein and the mRNA-like domain of transfer messenger RNA (tmRNA). J Biol Chem288, 30527-30542 (2013).
  10.  A. Svetlanov, N. Puri, P. Mena, A. Koller, A. W. Karzai, Francisella tularensis tmRNA system mutants are vulnerable to stress, avirulent in mice, and provide effective immune protection. Mol Microbiol85, 122-141 (2012).
  11.  P. Mehta, P. Woo, K. Venkataraman, A. W. Karzai, Ribosome purification approaches for studying interactions of regulatory proteins and RNAs with the ribosome. Methods Mol Biol905, 273-289 (2012).
  12.  M. Srinivasan et al., The highly conserved KEOPS/EKC complex is essential for a universal tRNA modification, t6A. EMBO J30, 873-881 (2011).
  13.  N. A. Okan, P. Mena, J. L. Benach, J. B. Bliska, A. W. Karzai, The smpB-ssrA mutant of Yersinia pestis functions as a live attenuated vaccine to protect mice against pulmonary plague infection. Infect Immun78, 1284-1293 (2010).
  14.  Z. Ge, P. Mehta, J. Richards, A. W. Karzai, Non-stop mRNA decay initiates at the ribosome. Mol Microbiol78, 1159-1170 (2010).
  15.  H. S. Fukuto, A. Svetlanov, L. E. Palmer, A. W. Karzai, J. B. Bliska, Global gene expression profiling of Yersinia pestis replicating inside macrophages reveals roles of a putative stress-induced operon in regulating type III secretion and intracellular cell division. Infect Immun, (2010).
  16.  Z. Ge, A. W. Karzai, Co-evolution of multipartite interactions between an extended tmRNA tag and a robust Lon protease in Mycoplasma. Mol Microbiol74, 1083-1099 (2009).
  17.  T. Sundermeier, Z. Ge, J. Richards, D. Dulebohn, A. W. Karzai, Studying tmRNA-mediated surveillance and nonstop mRNA decay. Methods Enzymol447, 329-358 (2008).
  18.  J. Richards, T. Sundermeier, A. Svetlanov, A. W. Karzai, Quality control of bacterial mRNA decoding and decay. Biochim Biophys Acta1779, 574-582 (2008).
  19.  T. R. Sundermeier, A. W. Karzai, Functional SmpB-ribosome interactions require tmRNA. J Biol Chem282, 34779-34786 (2007).
  20.  D. Dulebohn, J. Choy, T. Sundermeier, N. Okan, A. W. Karzai, Trans-translation: the tmRNA-mediated surveillance mechanism for ribosome rescue, directed protein degradation, and nonstop mRNA decay. Biochemistry46, 4681-4693 (2007).
  21.  J. S. Choy, L. L. Aung, A. W. Karzai, Lon protease degrades transfer-messenger RNA-tagged proteins. Journal of Bacteriology189, 6564-6571 (2007).
  22.  J. Richards, P. Mehta, A. W. Karzai, RNase R degrades non-stop mRNAs selectively in an SmpB-tmRNA-dependent manner. Mol Microbiol62, 1700-1712 (2006).
  23.  N. A. Okan, J. B. Bliska, A. W. Karzai, A Role for the SmpB-SsrA system in Yersinia pseudotuberculosis pathogenesis. PLoS Pathog2, e6 (2006).
  24.  P. Mehta, J. Richards, A. W. Karzai, tmRNA determinants required for facilitating nonstop mRNA decay. RNA12, 2187-2198 (2006).
  25.  D. P. Dulebohn, H. J. Cho, A. W. Karzai, Role of conserved surface amino acids in binding of SmpB protein to SsrA RNA. The Journal of biological chemistry281, 28536-28545 (2006).
  26.  T. R. Sundermeier, D. P. Dulebohn, H. J. Cho, A. W. Karzai, A previously uncharacterized role for small protein B (SmpB) in transfer messenger RNA-mediated trans-translation. Proc Natl Acad Sci U S A102, 2316-2321 (2005).
  27.  A. W. Karzai, R. T. Sauer, Protein factors associated with the SsrA.SmpB tagging and ribosome rescue complex. Proc Natl Acad Sci U S A98, 3040-3044 (2001).
  28.  S. Barends, A. W. Karzai, R. T. Sauer, J. Wower, B. Kraal, Simultaneous and functional binding of SmpB and EF-Tu-TP to the alanyl acceptor arm of tmRNA. J Mol Biol314, 9-21 (2001).
  29.  A. W. Karzai, E. D. Roche, R. T. Sauer, The SsrA-SmpB system for protein tagging, directed degradation and ribosome rescue. Nature Structural Biology7, 449-455 (2000).
  30.  J. F. Schildbach, A. W. Karzai, B. E. Raumann, R. T. Sauer, Origins of DNA-binding specificity: role of protein contacts with the DNA backbone. Proc Natl Acad Sci U S A96, 811-817 (1999).
  31.  R. Russell, A. Wali Karzai, A. F. Mehl, R. McMacken, DnaJ dramatically stimulates ATP hydrolysis by DnaK: insight into targeting of Hsp70 proteins to polypeptide substrates. Biochemistry38, 4165-4176 (1999).
  32.  A. W. Karzai, M. M. Susskind, R. T. Sauer, SmpB, a unique RNA-binding protein essential for the peptide-tagging activity of SsrA (tmRNA). EMBO J18, 3793-3799 (1999).
  33.  A. W. Karzai, R. McMacken, A bipartite signaling mechanism involved in DnaJ-mediated activation of the Escherichia coli DnaK protein. The Journal of biological chemistry271, 11236-11246 (1996).
  34.  B. Learn, A. W. Karzai, R. McMacken, Transcription stimulates the establishment of bidirectional lambda DNA replication in vitro. Cold Spring Harbor symposia on quantitative biology58, 389-402 (1993).
  35.  A. W. Karzai, W. W. Wright, Regulation of the synthesis and secretion of transferrin and cyclic protein-2/cathepsin L by mature rat Sertoli cells in culture. Biology of reproduction47, 823-831 (1992).
  36.  S. D. Zabludoff, A. W. Karzai, W. W. Wright, Germ cell-Sertoli cell interactions: the effect of testicular maturation on the synthesis of cyclic protein-2 by rat Sertoli cells. Biol Reprod43, 25-33 (1990).
  37.  W. W. Wright, S. D. Zabludoff, M. Erickson-Lawrence, A. W. Karzai, Germ cell-Sertoli cell interactions. Studies of cyclic protein-2 in the seminiferous tubule. Annals of the New York Academy of Sciences564, 173-185 (1989).