Completed Constructs

We have transformed Aurantiochytrium using an expression construct for zeocin resistance (shble) driven by Aurantiochytrium GAPDH promoter and terminator sequences – the GZG cassette – cloned into pUC19, with and without a copy of the Aurantiochytrium 18S rRNA gene. These two plasmids, pUC19_GZG and pUC19_18GZG respectively, are available at addgene

https://www.addgene.org/Jackie_Collier/

We also have replaced the shble gene with a yeGFP-shble fusion in the plasmid pUC19_18GeZG.