Graduate Research/Teaching Assistant                

August 2024-Present

State University of New York, Stony Brook University, Stony Brook, NY            

• Bioinformatics analysis on the correlation among neurodegeneration disease and osteodiseases
• Osteo organoids model development
• Cell mechanics: the impact of mechanical force stimulation
• Application of superparamagnetic nanoparticles on in vitro models
• Superparamagnetic and ferrimagnetic NP interaction with target proteins

Research staff                  

Oct 2023-May 2024

Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, NY  

• Establish iPSC platform for the pathogenesis of frontotemporal lobar degeneration (FTLD) research
  • iPSC maintenance and banking
  • differentiate iPSC into excitatory forebrain neurons and induced microglia
  • genetically edit iPSC for high-efficiency targeted differentiation
  • develop iPSC models and parallel compare to the mouse models/cell models
• Establish rAAV platform for in vivo study
  • Produce, purify, and analyze recombinant adeno-associated virus (rAAV)
  • gene delivery to study PGRN/TMEM106B in normal physiology vs pathogenesis of FTLD
• Functionally analyze PGRN within various cell models

 

Graduate Research Assistant

Aug 2021-Oct 2023

Department of Grain Science and Industry, Agriculture, Kansas State University, Manhattan, KS            

• Characterize iPSC culture in various conditions (2D, 2.5D, and 3D)
  • Maintained iPSC induced from human fibroblast and PBMC in 3D peptide hydrogel matrix culture system, and compared within different ECM mimetic systems (Matrigel, Col-gel, laminin, and vitronectin)
  • Analyzed pluripotency gene expression with modified 3D culture criteria among different iPS cell lines, culture media, and various experimental conditions
  • Isolated extracellular vesicles from 3D cultured iPSC, and quantified the size and concentration of each population via nanoparticle tracking analysis (NTA)
• Cell-engineered human foreskin fibroblast and PBMC into iPS cells in a 3D system (Sendai viral transfection), and optimize the reprogramming efficiency