Cohen, A. B., Novkov‐Bloom, A., Wesselborg, C., Yagudaeva, M., Aranguiz, E., & Taylor, G. T. (2021). Applying fluorescence in situ hybridization to aquatic systems with cyanobacteria blooms: Autofluorescence suppression and high‐throughput image analysis. Limnology and Oceanography: Methods.
Synopsis: Cyanobacterial Harmful Algal Blooms (CyanoHABs) are expanding geographically in both fresh and marine water bodies due to coastal eutrophication and global climate change and are restructuring the microbial ecology of these systems. Cyanobacterial autofluorescence can pose a significant impediment to accurately identifying prokaryotic taxonomic groups in environmental samples using fluorescence in situ hybridization (FISH). We apply novel fluorescence suppression chemistry and data filtration routine to FISH images that efficiently removes autofluorescent cells from final cell counts.
