Resume: TannviK_Resume

Graduate Research:

Microfluidics for Quantitative and Genomics Biology Laboratory

Principal Investigator: Eric Brouzes, PhD

Current Research Project: Advanced Digital PCR Device Development

As of August 2024, I am spearheading an innovative research project focused on the design and fabrication of a cutting-edge thermoplastic device for digital PCR sample partitioning. This advanced system aims to revolutionize nucleic acid amplification and quantification by efficiently dividing samples into 20,000 individual nanoliter chambers.

Project Objectives:

1. Address the limitations of current PDMS-based devices, particularly their high production costs.
2. Develop a cost-effective alternative using thermoplastics while maintaining high performance.
3. Solve the challenge of air entrapment during sample partitioning through innovative design.

Methodological Approach:

– Utilize thermoplastics and capillary effects to control the liquid-air interface.
– Explore the potential of Polycarbonate as a superior alternative to previously tested COC (Cyclic Olefin Copolymer).
– Implement a novel air displacement technique via capillary channels.
– Optimize sample partitioning reliability using micropipette technology.

Future Directions:

The next phase of this project will incorporate digital PCR (dPCR) methodologies, enabling:
– Absolute quantification of nucleic acids without calibration curves.
– Enhanced sample partitioning into numerous microreactors.
– Application of Poisson statistics for precise target sequence concentration determination.

This groundbreaking research is on track for publication in Spring 2025, promising significant advancements in the field of molecular diagnostics and research applications.

Musculoskeletal Research Laboratory

Principal Investigator: Mei Ete Chan, PhD

In my undergraduate studies, I formed a strong working relationship with Dr. Mei Ete Chan, which led to my involvement in her lab studying the effects of low-intensity vibration (LIV) on bone organoid formation. LIV, which applies mechanical oscillations to biological systems, operates through key parameters like amplitude, frequency, and treatment duration. While LIV’s anabolic effects have been well-documented in clinical and in vitro models, its impact on bone organoids was unexplored.                               In this pilot study, we sought to determine whether LIV could promote cell clustering, accelerate cell growth, and potentially trigger differentiation. By applying a frequency of 30Hz and an amplitude of 0.3 g over four weeks, we observed enhanced cell expansion and reduced apoptosis, suggesting LIV’s potential to stimulate tissue development. I contributed not only by learning to operate and troubleshoot the LIV device but also by helping to refine its design for more consistent signal delivery.                 Working with team members across different schedules highlighted the importance of clear communication and collaboration, skills that are vital in both academic and industrial research. This experience also strengthened my ability to design precise, controlled systems, which was crucial for optimizing LIV parameters and advancing our organoid research.

I am currently focused on two main projects:

1. Optimizing the Low-Intensity Vibration (LIV) device design:
I’m refining the LIV technology to be used in a portable, travel-friendly incubator. This improved design aims to facilitate easier sharing between laboratories, enhancing collaborative research opportunities.

2. Investigating LIV’s potential in T-cell activation for CAR-T therapy:
I’m conducting experiments using whole blood isolation techniques to extract and activate T-cells. The goal is to determine whether LIV stimulation can enhance the activation of CD28, a crucial co-stimulatory molecule. If successful, this could potentially improve the efficacy of CAR-T cell therapy, an innovative cancer treatment approach.