Renishaw inVia Confocal Raman Microscope

OLYMPUS DIGITAL CAMERA

The central research instrument is an inVia High Resolution Confocal Raman Microspectrometer, which includes:

  • a 633 nm He/Ne laser (17mW)
  • a 457/514 nm Ar ion laser (150mW)
  • a 785 nm diode laser (300mW)
  • 600, 1200, and 1800 l/mm spectrographic gratings and appropriate filters with > 30% optical throughput
  • a thermo-electric (TE) cooled 1024×256 CCD detector achieving a spectral resolution of 0.5 cm-1 @ 680 nm using the 1800 l/mm grating
  • main sampling platform is a Leica DM2500 upright microscope with computer-controlled motorized stage for XYZ mapping with <0.1 um step-size; 10x, 20x, 40x, 50x, 50xLWD, 63x and 100x objective lenses; operates in transmitted, reflected, bright field, dark field, epifluorescent, and Raman chemometric modes; DAPI, FITC, CY3, and CY5 fluorescent filter sets available; 150 mW Hg lamp for epifluorescence
  • Raman spatial resolution – diffraction limited to ~200 nm @ 457 nm and 100x magnification
  • Streamline spectrograph upgrade for rapid 2D and 3D Raman scans
  • Luminera Infinity 3 TE cooled camera for high-res video images and Renishaw camera for laser focusing
  • Linkam THMS600 Hot/Cold stage (-196 to +600°C) with T95 controller, enabling programmable local and remote control
  • video monitors, workstation and WiRE 4.1 software

OLYMPUS DIGITAL CAMERA785 nm diode, 457/514 nm Ar ion and 633 nm He-Ne lasers (from left to right)

OLYMPUS DIGITAL CAMERABeamsteer motors and mirrors

OLYMPUS DIGITAL CAMERASpectrograph

OLYMPUS DIGITAL CAMERACollimating lens, mirrors and focusing lens (below) and ultra-high precision spectrographic gratings (above)