Tiling light sheet microscopy of two membrane labeled mesodermal progenitors in the zebrafish tailbud. From Fu et al.

 

3D reconstruction of two membrane labeled mesodermal progenitors in the zebrafish tailbud. From Fu et al.

 

3D reconstruction of a zebrafish tailbud with ubiquitous nuclear label. From Fu et al.

 

Time lapse movies of transplants targeted to the midline progenitor population. The bottom is a “digitally straightened” embryo. Movies were used to retro-trace cells to create a midline progenitor fate map within the tailbud. From Row et al.

 

Transplanted wild-type cells (red) targeted to the midline progenitor population contribute to both the floor plate and notochord. The host embryo notochord is labeled in green. From Row et al.

 

Transplanted cells (red) with a heat-shock transgene that cell-autonomously inhibits the canonical Wnt signaling pathway contribute to the floor-plate but not the notochord. The host embryo notochord is labeled in green. From Row et al.