Osteoporosis, which is characterized as the loss of bone mass that consequently causes non-traumatic fractures in bones, poses a significant health threat in the elderly population.  Currently, the gold standard of the osteoporosis diagnostics is the dual energy x-ray absorptiometry (DXA), which measures bone mineral content (BMC) or bone mineral density (BMD). However, radiation and the high cost of the DXA device have limited its application in large medical facilities. Moreover, osteoporosis is not solely the result of losing bone mass. The bone quality parameters such as bone strength may also be associated with a significant fracture risk. Alternatively, ultrasound has become a new physical modality in the assessment of bone properties for the last two decades. Ultrasound wave propagation is a mechanical phenomenon and its characteristics are determined by the physical properties of the media including density and Young’s modulus. Currently, ultrasonic devices have been used as a preliminary diagnostic tool for osteoporosis in the clinical setting. Commercial ultrasound devices measure the acoustic properties at human calcaneus. It is a single point of measurement and is difficult to identify the distribution of bone properties such as density across the whole bone. Acoustic scan performs raster scanning measurements to obtain images similar to X-ray. Our scanning device can generate acoustic images of human calcaneus. It is composed of a two dimensional moving stage controlled by an embedded motion control system, co-axial ultrasound transmitter and receiver, and a desktop computer. The desktop computer controls the acoustic scanning, acquires ultrasound signal and performs data analysis. The system software was written in C++ and runs on Windows XP platform. The ultrasound images generated were ultrasound attenuation, broadband ultrasound attenuation and ultrasound velocity. This system was used in clinic test and NASA’s bed rest study.